Rat (H4IIE) and fish (PLHC-1) cell line bioassays for the detection of mixed function oxygenase inducers : methods description / J. Sherry ... [et al.]. : En13-5/97-06E-PDF

"In vitro cell-line bioassays for the detection of mixed function oxygenase (MFO) inducerssolve several practical problems associated with their whole fish counterparts. They are less costly, logistically simpler, and less labour intensive than whole fish tests. Many experiments that would be difficult using whole fish bioassays are made possible by in vitro bioassays. In vitro assays allow a higher sample throughput, as multiple tests can be run in a few microwell plates, and most in vitro techniques can be semi-automated by means of liquid handling devices and automated plate readers. Miniaturized in vitro bioassays radically reduce the amount of sample needed for MFO determinations, which permits tests of chemicals and samples that are expensive or available onlyin small amounts. In vitro bioassays are also ideal for testing small fractions from chromatographic separations of environmental samples or industrial effluents. The present report describes two in vitro bioassays based on rat (H411E) and fish (PLHC-'1) cell lines for the detection of MFO inducers in small volume environmental samples. The report is intended as a standard operating procedure that the researcher can use to assess the MFO inducing potency of pure chemicals, complex mixtures, or fractions from chromatographic separations"--Management perspective.

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