Protein-nucleic acid (receptor-ligand) binding detection techniques / Sara Sheibani, Nora W.C. Chan, DRDC - Suffield Research Centre. : D68-2/027-2018E-PDF

"The key component of the Inform Project WBE 5.3 is the development of a handheld biosensor to provide early warning to the Canadian Armed Forces (CAF) personnel for a potential biological threat. This technology is based on Toll-Like Receptors (TLRs) which are a class of proteins expressed by the innate immune system cells. TLRs recognize a specific component of pathogen through direct binding or via an intermediate molecule. Among the 10 TLRs that have been identified to date, TLRs 3, 7, 8, and 9 recognize various types of microbial nucleic acids. There are two main types of nucleic acids including deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). Many of the emerging infectious diseases are caused by single-stranded ribonucleic acid (ssRNA) viruses. Based on the Baltimore classification, viruses are classified into families depending on their type of nucleic acid and method of replication. The ssRNA viruses belong to class IV or V. A part of TLR biosensor project is on detection of ssRNA viruses using TLR7 and TLR8 through a binding assay. Therefore, the purpose of this Scientific Report is to recommend the best available technique to study the binding of TLR7 and TLR8 (receptor) with ssRNA virus (ligand) in the context of this project. To achieve this goal, in the first step, a literature review on the current techniques is required. Among a small set of current methods (labelling and label-free), Surface Plasmon Resonance (SPR) which is a label-free technique is recommended for the purpose of this project"--Abstract, page i.

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