Biological test method : test for survival, growth and reproduction in sediment and water using the freshwater amphipod Hyalella azteca / Method Development and Applications Unit, Science and Technology...: En49-7/1-33-2017E-PDF

"Revised methods now recommended by Environment and Climate Change Canada for performing sediment and water-only toxicity tests using the freshwater amphipod Hyalella azteca are described in this report. The endpoints for these tests are survival and dry weight of amphipods at the end of a 14-day test. This method also includes a reproduction endpoint over a longer term exposure (42 days). Key to successfully conducting the longer-term test is the recovery from sediment and accurate counting of the young of the adults at the end of the 42 day test, and as such, the 42-d methods include a performance criterion that requires laboratories to meet a specified % recovery. This revised version of Report RM/33 supersedes Environment and Climate Change Canada’s test for survival and growth in sediment using Hyalella azteca, which was published as Report EPS 1/RM/33 in January 2013. The sediment test is intended primarily for measuring the adverse effect(s) of freshwater sediments, although procedures for testing estuarine sediments (≤15‰ salinity) are also described”--Abstract, p. ii.

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